Instruments
The Resource makes use of instrumentation and equipment in the Wysocki, Russell, Olesik, and Holland Research labs, the Campus Chemical Instrument Center (CCIC) at OSU, and the Laboratory for Biological Mass Spectrometry (LBMS) at Texas A&M.
Waters Synapt G2 HDMS
Ion mobility enables gas-phase ions to be separated by their size, shape, and charge. This affords a dimension of separation orthogonal to the standard m/z analysis. The instrument has been modified with a custom surface-induced dissociation (SID) device which can be placed before the IM region (SID-IM), after the IM region (IM-SID), or on either side of the IM cell to allow for SID-IM-SID. Collision-induced dissociation can also be performed before or after the IM cell, even with SID installed. This instrument is mainly used for studies of large protein complexes. In addition, we have a linear field ion mobility cell that can be installed and used to make direct collision cross section measurements without the need for calibration, as is necessary for TWIMS measurements.
Waters Synapt G2S HDMS
A newer generation of Waters Synapt G2 instrument with improved “step-wave” design after the ion source for improved sensitivity. Like the G2 this instrument has been modified with a custom surface-induced dissociation (SID) device which can be placed before the IM region (SID-IM), after the IM region (IM-SID), or on either side of the IM cell to allow for SID-IM-SID. Collision-induced dissociation can also be performed before or after the IM cell, even with SID installed. This instrument is equipped with electron transfer dissociation (ETD) and has also been modified to incorporate a 193 nm laser for ultraviolet photo dissociation (UVPD). It is mainly used for studies of large protein complexes. This instrument also has a linear field ion mobility cell that can be installed and used to make direct collision cross section measurements without the need for calibration.
Thermo Scientific Exactive Plus EMR
A benchtop Orbitrap instrument with extended mass range (EMR) which enables analysis of large molecules (m/z 350-20,000) with high mass accuracy and is equipped with a variable pressure higher-energy collision-induced dissociation (HCD) cell. This instrument has been modified to include a high-mass range quadrupole mass filter and a custom surface-induced dissociation (SID) device. The EMR is primarily used for analysis of proteins and protein complexes by both direct infusion and online non-denaturing separation techniques.
Campus Chemical Instrument Center
Campus Chemical Instrument Center (CCIC) is a university core facility housed administratively under the Office of Research, which is headed by Ohio State’s Vice- President for Research. CCIC offers mass spectrometry and proteomics and nuclear magnetic resonance (NMR) services to internal and external customers. Embedded within the center is also the Proteomics Core of the NIH-funded Comprehensive Cancer Center. CCIC Mass Spectrometry and Proteomics provides traditional proteomics services to cancer center members and other investigators, using the CCIC instrumentation and staff. CCIC provides support to the P41 resource through access to ultra-high resolution instrumentation such as the 15T FT-ICR, as well as instrumentation for complimentary experiments such as crosslinking and bottom-up studies.